CLOCKSHIFT

Breast cancer risk and epigenetic effects of the rotating night shift work and lifestyle.

WP3

Title: Night shift work, lifestyle factors and methylation status of selected genes involved in breast carcinogenesis

Leader: Prof. Beata Pepłońska, MD, PhD

Time: From 1 to 36 months

The aim of this Work package is to assess the association between rotating night shift, lifestyle factors and epigenetic (methylation) changes of tumor suppressor genes in the nurses and midwives,. Data from the cross-sectional study of the nurses and midwives (n=725 women) (original study funded from a PNRF grant) will be used for this purpose. During the original study data on occupational history and potential confounders were collected including information about lifestyle factors: diet (FFQ), physical activity (IPAQ), smoking, alcohol consumption. Weight and height were measured, BMI calculated. Determination of methylation of tumor suppressor genes important in breast tumorigenesis (TP53, CDKN1A, CDKN2A, RB1, BRCA1, BRCA2) will be performed during current project and is described under WP1. Epidemiological analyses will be performed at the Epidemiology Unit of the Nofer Institute addressing the following issues: diet (folates intake in specific), alcohol consumption, smoking, physical activity, and obesity. Food frequency questionnaire data will be used for determination of the variability between study subjects in folates intake, since dietary folates intake has been recognized as major confounder for analyses of genes methylation.