CLOCKSHIFT

Breast cancer risk and epigenetic effects of the rotating night shift work and lifestyle.

WP3

Night shift work, lifestyle factors and methylation status of selected genes involved in breast carcinogenesis

Leader: Msc Weronika Burdelak

The aim of this work package is to assess the association between rotating night shift work, lifestyle factors and epigenetic (methylation) changes of tumor suppressor genes in nurses and midwives,. Data from a cross-sectional study of nurses and midwives (n=725 women) will be used (for more information, see "Study population"). During the original study data on occupational history and potential confounders were collected including information about lifestyle factors: diet, physical activity, smoking, alcohol consumption. Weight and height were measured and the BMI was calculated. Determination of the methylation of tumor suppressor genes important in breast tumorigenesis (TP53, CDKN1A, CDKN2A, RB1, BRCA1, BRCA2) is described under WP1. Epidemiological analyses addressing diet (particular folate intake), alcohol consumption, smoking, physical activity, and obesity will be performed at the Epidemiology Unit of the Nofer Institute. Folate intake has been recognized as a major confunder for methylation analysis. Food frequency questionnaire data will be used for determination of the variability in folate intake between study subjects.