Breast cancer risk and epigenetic effects of the rotating night shift work and lifestyle.


Night shift work, chronotype, sleep disturbances and methylation status of core circadian genes and selected cell cycle regulatory genes

Leader: MSc Agnieszka Bukowska

The aim of this work package is to assess association between night shift work, sleep disturbances and methylation status of circadian rhythm genes (BMAL1, CLOCK, Per1, Per2, Per3, Cry1, Cry 2, NPAS2) and selected cell cycle regulatory genes (TP53, CDKN1A, CDKN2A, RB1, BRCA1, BRCA2). Since chronotype is essential for the analysis of sleep disturbances, validated tools for chronotype assessment (for example the Morningness-Eveningness Questionare) will be used during interviews with the participating women. The Pittsburg Sleep Quality Questionnaire will be used to analyze association between night shift work and sleep quality. In addition, data collected via one-week diary will be used. DNA has been isolated from peripheral blood leukocytes. Determination of circadian rhythm gene methylation and cell cycle regulatory gene methylation methylation is described under WP1. Epidemiological analyses will be performed at the epidemiology unit of the Nofer Institute of Occupational Medicine.